Volume 37, Number 4
 
 
 
Archive
 
+ Volume 37, 2018
Issue 1
Issue 2
Issue 3
Issue 4
+ Volume 36, 2017
+ Volume 35, 2016
+ Volume 34, 2015
+ Volume 33, 2014
+ Volume 32, 2013
+ Volume 31, 2012
Science, Technology and Development Vol. 37 (4), 2018
Perspective
Study of Phylogenetic Relationship among Vigna Species Using Morphological Characters and Seed Storage Proteins
Ali Asghar, Muhammad Kashif Ilyas, Asif Javaid, Murad Ali, Waqar Khan, Manzoor Hussain and Abdul Ghafoor
 
Abstract: The experiment was conducted to study the phylogenetic relationship among four cultivated Vigna species, including Vigna radiata, Vigna mungo, Vigna aconitifolia and Vigna unguiculata, using the germplasm from National Genebank of Pakistan. Seed of 15 accessions of each species was taken from Genebank of Plant Genetic Resources Institute, NARC, Islamabad, and these 60 accessions were planted in the NARC field, using augmented design. Data was recorded for eight morphological characters, including branching pattern, calyx color, corolla color, flowering period, leaf senescence, petiole color, petiole length and twining tendency. Cluster analysis was conducted, using software Statistica through Ward’s method. The seed of all 60 accessions was subjected to SDS-PAGE analysis. Cluster analysis of seed storage proteins was conducted using Unweighted Pair-group Average method. Cluster analysis for morphological characters divided the accessions into four clusters of different species. In case of seed storage proteins, 13 clusters were observed, five of which included accessions of different species. It was found by cluster analysis for morphological characters that Vigna radiata and Vigna mungo are in same major cluster at linkage and they are related to Vigna unguiculata at greater linkage distance. Phylogenetic tree constructed on the basis of morphological characters indicates genetic relationship among cultivated Vigna species. Seed storage protein analysis gives an estimate of diversity in protein profile and the possible genetic relationship.
 
      Fulltext PDF  | 
 
 
SIGN IN
  User ID
 
  Password
 
 
  Forgot Password?   |   Register Now
 
   Quick Links
  About the journal
  Current issue
   Archive
  Editorial board
  Submit manuscript
  Abstracting and indexing
  Article submission
  Guide to authors
  For subscribers
  Policies
 
 
Science, Technology and Development © 2019                                                                                                                                   Maintained and Developed by ansinet